7ASI
Fixed-target serial femtosecond crystallography using in cellulo grown Neurospora crassa HEX-1 microcrystals. (Chips 1+2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE MFX |
| Synchrotron site | SLAC LCLS |
| Beamline | MFX |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2017-10-02 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.312 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 58.730, 58.730, 192.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.365 - 1.704 |
| R-factor | 0.2225 |
| Rwork | 0.219 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1khi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.316 |
| Data reduction software | Cheetah |
| Data scaling software | CrystFEL (v0.6.3) |
| Phasing software | PHASER (v1.16-3546) |
| Refinement software | PHENIX (v1.16-3546) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 1.744 |
| High resolution limit [Å] | 1.704 | 1.704 |
| Number of reflections | 22635 | 1469 |
| <I/σ(I)> | 5.421 | 0.7 |
| Completeness [%] | 100.0 | 99.86 |
| Redundancy | 124.12 | 16.4 |
| CC(1/2) | 0.964 | 0.160 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 300.15 | Neurospora crassa HEX-1 P3 rBV-infected Sf9 cells at multiplicity of infection 1, cultivated in serum-free ESF921 medium |
