7AR0

Co-Crystal Structure of Variant Surface Glycoprotein VSG2 in complex with Nanobody VSG2(NB19)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-19
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 41 2 2
Unit cell lengths	141.738, 141.738, 89.582
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.726 - 2.139
R-factor	0.2071
Rwork	0.206
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vsg 5lhr(ChainB)
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (3.25)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.726	2.216
High resolution limit [Å]	2.139	2.139
Number of reflections	50820	4971
<I/σ(I)>	8.7
Completeness [%]	99.8
Redundancy	26.7
CC(1/2)	0.997	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	0.1 M ADA pH 6.5, 2 M Ammonium Sulfate, 0.1 MgSO4