7AQY

Co-Crystal Structure of Variant Surface Glycoprotein VSG2 in complex with Nanobody VSG2(NB11)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-19
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	82.644, 97.749, 104.506
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.024 - 1.900
R-factor	0.2107
Rwork	0.209
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vsg 5lhr(chainB)
Data reduction software	xia2 (3.25)
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.024	1.968
High resolution limit [Å]	1.900	1.900
Number of reflections	67339	6654
<I/σ(I)>	14.76
Completeness [%]	99.8
Redundancy	10.8
CC(1/2)	0.987	0.187

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	0.1 M Imidazole, 19% PEG 1500