7AQB

Crystal structure of human mitogen activated protein kinase 6 (MAPK6)

Replaces:  2I6L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9791
Spacegroup name	P 43 21 2
Unit cell lengths	83.317, 83.317, 181.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.880 - 2.250
R-factor	0.2242
Rwork	0.222
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1erk
RMSD bond length	0.008
RMSD bond angle	1.460
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.880	48.880	2.320
High resolution limit [Å]	2.250	9.000	2.250
Rmerge	0.156	0.098	2.484
Rmeas	0.164	0.108	2.613
Rpim	0.050	0.041	0.802
Total number of observations		5255	28538
Number of reflections	31175	594	2803
<I/σ(I)>	10.6	23.8	2.1
Completeness [%]	100.0	99.5	100
Redundancy	10.7	8.8	10.2
CC(1/2)	0.857	0.867	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	277	1.8M MgSO4; 0.1M MES