7AMN

Structure of LuxR with DNA (repression)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9790
Spacegroup name	P 41
Unit cell lengths	69.339, 69.339, 123.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.030 - 2.300
R-factor	0.2115
Rwork	0.210
R-free	0.24240
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.822
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.603	2.244
High resolution limit [Å]	2.236	2.236
Number of reflections	28010	280
<I/σ(I)>	15.3
Completeness [%]	99.7
Redundancy	5
CC(1/2)	0.990	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	50mM Sodium Formate, 50mM Tris PH7.5, 22% PEG 2000MME