7ALS

Alpha and beta Lactose isomer structure of Galectin 8, N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	54.560, 62.540, 84.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.150 - 1.350
R-factor	0.15181
Rwork	0.149
R-free	0.19876
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gzd
RMSD bond length	0.016
RMSD bond angle	1.972
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.500	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.046
Number of reflections	60843	3044
<I/σ(I)>	18.8
Completeness [%]	99.8
Redundancy	9.2
CC(1/2)	0.999	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.5	24% (w/v) PEG 2000 MME