7ALK
Structure of Drosophila C2-DSL-EGF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.990, 86.736, 47.558 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 29.110 - 3.000 |
| R-factor | 0.2437 |
| Rwork | 0.241 |
| R-free | 0.29680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mvx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.583 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.110 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 5066 | 520 |
| <I/σ(I)> | 4.55 | 0.85 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.952 | 0.483 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris pH 8.5, 0.2M MgCl2, 30% PEG4K |
