7AL2
Cell division protein SepF from Methanobrevibacter smithii in complex with FtsZ-CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.847, 64.847, 107.924 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.910 - 2.701 |
| R-factor | 0.2305 |
| Rwork | 0.229 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7al1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.910 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.076 | 0.702 |
| Number of reflections | 3807 | 493 |
| <I/σ(I)> | 15.4 | 2.7 |
| Completeness [%] | 95.7 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M ammonium sulfate, 30% PEG 8K |
