7AL1
Cell division protein SepF from Methanobrevibacter smithii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9875 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.053, 53.053, 53.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.970 - 1.400 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb code 3ZIE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.057 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.950 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.056 | 1.029 |
| Number of reflections | 17704 | 846 |
| <I/σ(I)> | 27.1 | 3.4 |
| Completeness [%] | 99.9 | |
| Redundancy | 19.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M TRIS, pH 8.5, 30% PEG 10K |
