7AKY
Crystal structure of the viral rhodopsin OLPVR1 in P21212 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 46.525, 115.796, 53.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.1451 |
| Rwork | 0.143 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sqg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.590 | 48.590 | 1.420 |
| High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
| Rmerge | 0.072 | 0.063 | 1.810 |
| Rmeas | 0.078 | 0.071 | 1.972 |
| Rpim | 0.031 | 0.032 | 0.775 |
| Total number of observations | 364526 | 2002 | 17904 |
| Number of reflections | 57845 | 421 | 2820 |
| <I/σ(I)> | 12.3 | 33 | 1 |
| Completeness [%] | 100.0 | 98.6 | 99.9 |
| Redundancy | 6.3 | 4.8 | 6.3 |
| CC(1/2) | 0.998 | 0.995 | 0.453 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8.2 | 293 | 10 mM CaCl2, 10 mM MgCl2, 24% PEG 6000, 100mM Tris (pH 8.2), monopalmitolein |
