7AKN
Thermolysin from Bacillus thermoproteolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 92.408, 92.408, 129.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.980 - 2.460 |
| R-factor | 0.1583 |
| Rwork | 0.155 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kei |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.503 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.068 | 0.350 |
| Rmeas | 0.074 | 0.470 |
| Number of reflections | 12427 | 1769 |
| <I/σ(I)> | 8.9885 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | pH 7.5 |
