7AK8
Structure of Salmonella TacT1 toxin bound to TacA1 antitoxin C-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 |
| Unit cell lengths | 96.140, 96.140, 120.638 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.850 - 2.500 |
| R-factor | 0.2164 |
| Rwork | 0.214 |
| R-free | 0.26780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fvj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.377 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.850 | 48.860 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.128 | ||
| Rmeas | 0.138 | 0.063 | 0.970 |
| Rpim | 0.050 | 0.023 | 0.352 |
| Total number of observations | 16143 | 15873 | |
| Number of reflections | 43178 | 2181 | 2149 |
| <I/σ(I)> | 8.4 | 23 | 1.6 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 7.5 | 7.4 | 7.4 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 1.0 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate |
