7AK7
Structure of Salmonella TacT2 toxin bound to TacA2 antitoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.491, 54.452, 76.900 |
| Unit cell angles | 100.57, 97.73, 117.00 |
Refinement procedure
| Resolution | 46.660 - 2.140 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fvj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.385 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.220 | 73.260 | 2.180 |
| High resolution limit [Å] | 2.140 | 5.810 | 2.140 |
| Rmerge | 0.073 | ||
| Rmeas | 0.103 | 0.047 | 0.726 |
| Rpim | 0.073 | 0.033 | 0.514 |
| Total number of observations | 3273 | 3103 | |
| Number of reflections | 36519 | 1868 | 1738 |
| <I/σ(I)> | 3.8 | 7.1 | 1.2 |
| Completeness [%] | 97.1 | 99 | 92.2 |
| Redundancy | 1.8 | 1.8 | 1.8 |
| CC(1/2) | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.01M Magnessium chloride hexahydrate 0.05M MES monohydrate 1.8M Lithium sulfate monohydrate |
