7AIK
Ribonucleotide Reductase R2 protein from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.420, 69.420, 177.973 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.370 - 2.100 |
| R-factor | 0.21948 |
| Rwork | 0.217 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ci4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.537 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 26676 | 1295 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.990 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M citrate pH 4.0, 1.0 M lithium chloride, 20 % PEG6000 |
