7AIE
Crystal structure of a truncated form of the KLC1-TPR domain ([A1-B5] fragment) - Monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.490, 72.710, 110.870 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 46.330 - 3.287 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oj8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.330 | 3.480 |
| High resolution limit [Å] | 3.287 | 3.287 |
| Number of reflections | 17338 | 2626 |
| <I/σ(I)> | 4.55 | 0.93 |
| Completeness [%] | 98.6 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.991 | 0.537 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 20% PEG8000, 0.6M magnesium acetate, 50mM sodium cacodylate pH 6.5 |
