7AHW
The crystal structure of gene product PA4063 from Pseudomonas aeruginosa
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000100 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.825, 65.825, 101.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.324 - 1.952 |
| Rwork | 0.193 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7aly |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.835 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.081 | 0.037 |
| Rmeas | 0.088 | 0.040 |
| Rpim | 0.035 | 0.015 |
| Number of reflections | 16839 | 1154 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.7 | 8.9 |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | ammonium sulfate 2M, sodium chloride 2M |
