7AHW

The crystal structure of gene product PA4063 from Pseudomonas aeruginosa

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000100
Spacegroup name	P 41 21 2
Unit cell lengths	65.825, 65.825, 101.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.324 - 1.952
Rwork	0.193
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7aly
RMSD bond length	0.014
RMSD bond angle	1.835
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.550	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.081	0.037
Rmeas	0.088	0.040
Rpim	0.035	0.015
Number of reflections	16839	1154
<I/σ(I)>	17.3
Completeness [%]	99.9
Redundancy	11.7	8.9
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	ammonium sulfate 2M, sodium chloride 2M