7AHT
Structure of the N-domain of the K+/H+ antiporter subunit KhtT at pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978566 |
Spacegroup name | P 43 |
Unit cell lengths | 48.524, 48.524, 66.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.312 - 2.160 |
R-factor | 0.2468 |
Rwork | 0.245 |
R-free | 0.27940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7agv |
RMSD bond length | 0.002 |
RMSD bond angle | 0.501 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.520 | 2.230 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.048 | 1.207 |
Rmeas | 0.050 | 1.252 |
Rpim | 0.014 | 0.333 |
Number of reflections | 8366 | 734 |
<I/σ(I)> | 22.4 | 2.4 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 13.6 | 14.1 |
CC(1/2) | 1.000 | 0.943 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES pH 7.5 1.6 M Ammonium sulfate |