7AHT

Structure of the N-domain of the K+/H+ antiporter subunit KhtT at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978566
Spacegroup name	P 43
Unit cell lengths	48.524, 48.524, 66.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.312 - 2.160
R-factor	0.2468
Rwork	0.245
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7agv
RMSD bond length	0.002
RMSD bond angle	0.501
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.520	2.230
High resolution limit [Å]	2.160	2.160
Rmerge	0.048	1.207
Rmeas	0.050	1.252
Rpim	0.014	0.333
Number of reflections	8366	734
<I/σ(I)>	22.4	2.4
Completeness [%]	99.8	99.4
Redundancy	13.6	14.1
CC(1/2)	1.000	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM HEPES pH 7.5 1.6 M Ammonium sulfate