7AH1
L19 diabody fragment from immunocytokine L19-IL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.810, 91.138, 99.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.450 - 2.000 |
| Rwork | 0.198 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gs1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.767 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.142 | 1.163 |
| Rpim | 0.100 | 0.827 |
| Number of reflections | 17195 | 1241 |
| <I/σ(I)> | 7.5 | 1.3 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 5.3 | 5.4 |
| CC(1/2) | 0.995 | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 10 mg/ml in 100 mM HEPES/NaOH, 10% PEG 6000, pH 7.0 |
