7AGZ
BsrV no-histagged
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.030, 82.090, 160.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.200 - 1.520 |
| R-factor | 0.1858 |
| Rwork | 0.185 |
| R-free | 0.20030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4beu |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.563 |
| Data reduction software | Aimless |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.200 | 1.570 |
| High resolution limit [Å] | 1.516 | 1.516 |
| Rmerge | 0.092 | 0.562 |
| Rpim | 0.036 | 0.205 |
| Number of reflections | 111214 | 10958 |
| <I/σ(I)> | 14 | 4.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.2 | |
| CC(1/2) | 0.981 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.1 M Bis-Tris propane pH 7.5, 0.2 M Sodium Iodide, and 24% (p/v) of PEG 3350 |
