7AGM

Crystal structure of the N-acetylmuramyl-L-alanine amidase, Ami1, from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-03
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	41.850, 68.840, 63.720
Unit cell angles	90.00, 91.87, 90.00

Refinement procedure

Resolution	46.750 - 1.350
R-factor	0.1571
Rwork	0.156
R-free	0.18060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7agl
RMSD bond length	0.005
RMSD bond angle	0.769
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.600	1.390
High resolution limit [Å]	1.350	1.350
Rmeas	0.060	1.400
Number of reflections	79198	8163
<I/σ(I)>	15.1
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Bis-Tris pH 5.5, 17% (w/v) PEG 3350