Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.79 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.914, 81.357, 91.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.102 - 0.792 |
| Rwork | 0.121 |
| R-free | 0.13190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sil |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.468 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.107 | 0.810 |
| High resolution limit [Å] | 0.792 | 0.792 |
| Rmeas | 0.061 | 1.572 |
| Number of reflections | 355798 | 19346 |
| <I/σ(I)> | 23.61 | 0.88 |
| Completeness [%] | 94.7 | 70.1 |
| Redundancy | 3.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | Crystals grown by hanging drop vapour diffusion. A 1:1 mixture of protein solution and an 8:4 mixture of K2HPO4 to KH2PO4 was placed above a well of an 8:6 solution of K2HPO4 to KH2PO4. Then serially cryoprotected in situ to 40% glycerol (v/v with mother liquor) in 10% increments over a period of a few minutes. |
