7AF1

The structure of Artemis/SNM1C/DCLRE1C with 2 Zinc ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976
Spacegroup name	P 1
Unit cell lengths	35.752, 47.973, 48.153
Unit cell angles	82.68, 76.35, 85.81

Refinement procedure

Resolution	47.530 - 1.700
R-factor	0.19156
Rwork	0.191
R-free	0.21169
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tt5
Data reduction software	DIALS
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.530	47.530	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.132	0.087	0.795
Total number of observations	117157	813	5983
Number of reflections	33010	225	1686
<I/σ(I)>	5.8	12	2
Completeness [%]	97.4	99.6	95.6
Redundancy	3.5	3.6	3.5
CC(1/2)	0.986	0.979	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	20% PEG 3350 0.3 M Ammonium Chloride