7AED

VirB8 domain of PrgL from Enterococcus faecalis pCF10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	H 3
Unit cell lengths	124.521, 124.521, 66.426
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.740 - 1.753
R-factor	0.18
Rwork	0.179
R-free	0.19810
Structure solution method	SIRAS
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.860	1.860
High resolution limit [Å]	1.753	1.753
Rmeas	0.050
Number of reflections	76794	12242
<I/σ(I)>	16.3
Completeness [%]	99.6
Redundancy	5.3
CC(1/2)	0.990	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	bis-Tris, PEG 3350