7ADS

Orf virus Apoptosis inhibitor ORFV125

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 62 2 2
Unit cell lengths	106.613, 106.613, 60.589
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.656 - 2.220
R-factor	0.244911480668
Rwork	0.244
R-free	0.26039
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ORFV_Bax
RMSD bond length	0.003
RMSD bond angle	0.540
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.660	2.290
High resolution limit [Å]	2.220	2.220
Rmerge	0.100	0.100
Number of reflections	10487	929
<I/σ(I)>	14.2
Completeness [%]	100.0
Redundancy	19.2
CC(1/2)	1.000	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M Sodium citrate tribasic dehydrate 0.02M magnesium chloride 0.1M Bis tris propane 20% PEG 3350