7ADQ
Serial Laue crystallography structure of dehaloperoxidase B from Amphitrite ornata
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-04-19 |
| Detector | RAYONIX MX340-HS |
| Wavelength(s) | 0.815-0.863 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.800, 67.300, 67.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.660 - 2.010 |
| R-factor | 0.165 |
| Rwork | 0.162 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ixf |
| Data reduction software | Precognition |
| Phasing software | REFMAC |
| Refinement software | PHENIX (v1.1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | |
| Number of reflections | 12928 | 513 |
| <I/σ(I)> | 34.1 | |
| Completeness [%] | 67.8 | 22 |
| Redundancy | 7.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6 | 277 | 30 mg per ml DHP mixed with 200 mM ammonium sulfate 40 percent PEG 4000, in a 1 to 4 ratio in a total volume of 250 to 500 microlitres |
