7ADI
KirBac3.1 W46R: role of a highly conserved tryptophan at the membrane-water interface of Kir channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07114 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.770, 113.980, 89.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.020 - 2.800 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.28730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xl6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.594 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.340 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.830 |
| Rmerge | 0.060 | 1.551 |
| Number of reflections | 189833 | 29583 |
| <I/σ(I)> | 19.67 | 1.31 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 7.1 | |
| CC(1/2) | 1.000 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 15% (w/v) PEG- MME 5k, 15% (v/v) glycerol, 1M Lithium Chloride, and 100 mM MES pH 6.5 |
