7AD5
Crystal structure of the effector AvrLm5-9 from Leptosphaeria maculans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.240, 57.240, 119.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.340 - 2.140 |
| R-factor | 0.2251 |
| Rwork | 0.223 |
| R-free | 0.27550 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.506 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.340 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 1.840 | |
| Rmeas | 0.231 | |
| Number of reflections | 11526 | 1742 |
| <I/σ(I)> | 8.6 | 1.1 |
| Completeness [%] | 99.4 | 96.3 |
| Redundancy | 12.49 | |
| CC(1/2) | 0.997 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.7M sodium acetate, 80mM nickel sulfate, 0.1M Hepes pH7.5 |
