7ACX

H/L (SLPH/SLPL) complex from C. difficile (R7404 strain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9282
Spacegroup name	P 1 21 1
Unit cell lengths	78.052, 137.940, 84.734
Unit cell angles	90.00, 100.70, 90.00

Refinement procedure

Resolution	83.260 - 2.650
R-factor	0.228
Rwork	0.225
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cvz 5j6q D_129110990/992/995
RMSD bond length	0.007
RMSD bond angle	1.185
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.260	2.750
High resolution limit [Å]	2.650	2.650
Number of reflections	51166	5098
<I/σ(I)>	12
Completeness [%]	99.7
Redundancy	48
CC(1/2)	0.712	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.0M LiCl2 12% PEG 6000 0.1M MES pH6.5 10%Glyc