7ACG
In meso structure of the alginate exporter, AlgE, from Pseudomonas aeruginosa in 9.8 monoacylglycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.751, 74.187, 114.992 |
| Unit cell angles | 90.00, 102.73, 90.00 |
Refinement procedure
| Resolution | 44.370 - 1.850 |
| R-factor | 0.1855 |
| Rwork | 0.184 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4afk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.051 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.370 | 1.916 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 39482 | 3904 |
| <I/σ(I)> | 8.35 | 1.36 |
| Completeness [%] | 99.1 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.990 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | PEG400, lithium sulphate, ammonium sulphate, sodium citrate |
