7ACC

human GTP cyclohydrolase I feedback regulatory protein (GFRP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9998
Spacegroup name	P 43 21 2
Unit cell lengths	107.270, 107.270, 142.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.810 - 2.040
R-factor	0.1685
Rwork	0.167
R-free	0.19080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jg5
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.810	2.120
High resolution limit [Å]	2.040	2.040
Number of reflections	53417	5243
<I/σ(I)>	26.8
Completeness [%]	99.9
Redundancy	3.6
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	2.4 M di-Sodium malonate; pH 7.0 (JCSG F9)