7AC2

Structure of Hen Egg White Lysozyme collected by rotation serial crystallography on a COC membrane at a synchrotron source

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	79.020, 79.020, 37.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.510 - 1.507
R-factor	0.1815
Rwork	0.180
R-free	0.20870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ne0
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.510	1.540
High resolution limit [Å]	1.500	1.500
Number of reflections	19098	1880
<I/σ(I)>	10.44	0.44
Completeness [%]	99.7
Redundancy	26.5
CC(1/2)	0.997	0.172

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	dissolved 50 mg/ml in 100 mM Na Acetate pH 3.0. mixed 1:1 (v:v) with 19.04% NaCl, 5.44% PEG 8000, 68 mM Na acetate pH 3.0