Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 140.016, 156.570, 112.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.394 - 2.177 |
| Rwork | 0.173 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qyj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.759 |
| Data reduction software | XDS (VERSION Mar 15, 2019) |
| Data scaling software | XDS (VERSION Mar 15, 2019) |
| Phasing software | PHASER (Version: 2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.310 |
| High resolution limit [Å] | 2.177 | 2.177 |
| Rmeas | 0.220 | 1.535 |
| Number of reflections | 129462 | 20548 |
| <I/σ(I)> | 9.1 | 1.3 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 6.7 | 6.4 |
| CC(1/2) | 0.994 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2 uL reservoir (16% PEG4000, 0.1 M Tris pH 9.5, 0.1 M NaOAc, 0.018 M imidazol pH 7.0) + 2 uL protein solution (10 mg/ml protein, 10 mM Tris pH 7.5, 20 mM NaCl) + 0.66 uL extra (1 M imidazol pH 6.5) |
