7ABM

X-ray structure of phosphorylated Barrier-to-autointegration factor (BAF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	70.320, 70.320, 40.625
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.160 - 3.004
R-factor	0.2076
Rwork	0.196
R-free	0.32610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ci4
RMSD bond length	0.009
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	BUSTER (2.10.3 (19-MAR-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	3.180
High resolution limit [Å]	3.000	3.000
Number of reflections	2463	390
<I/σ(I)>	7.8
Completeness [%]	100.0	100
Redundancy	19.4
CC(1/2)	0.998	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	pBAF was crystallized by the hanging drop method against 0.2 M sodium fluoride, 20% w/v PEG 3350