7ABA
The structure of the Bottromycin biosynthetic protein SalCYP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.738 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.448, 97.403, 93.285 |
| Unit cell angles | 90.00, 104.58, 90.00 |
Refinement procedure
| Resolution | 48.825 - 1.850 |
| R-factor | 0.143185800858 |
| Rwork | 0.141 |
| R-free | 0.17574 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.779 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.825 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.158 | 0.699 |
| Number of reflections | 74132 | 7163 |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 28.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG3350, Calcium acetate |
