7AB6
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) I52T variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 53.866, 110.784, 39.094 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.490 - 1.900 |
| R-factor | 0.1813 |
| Rwork | 0.180 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rhf |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.222 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.490 | 48.440 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.152 | 0.040 | 0.913 |
| Rmeas | 0.164 | 0.043 | 0.977 |
| Rpim | 0.059 | 0.016 | 0.346 |
| Total number of observations | 1360 | 9259 | |
| Number of reflections | 19058 | 218 | 1199 |
| <I/σ(I)> | 9.3 | 26.8 | 2.2 |
| Completeness [%] | 99.6 | 99.4 | 100 |
| Redundancy | 7.4 | 6.2 | 7.7 |
| CC(1/2) | 0.996 | 0.999 | 0.824 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Lithium sulfate 0.1M BIS-Tris pH 5.5 25% PEG 3350 |
