7AAS
Crystal structure of nitrosoglutathione reductase (GSNOR) from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.832, 143.002, 206.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.973 - 1.800 |
| R-factor | 0.1749 |
| Rwork | 0.173 |
| R-free | 0.20450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dla |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.973 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.533 |
| Rmeas | 0.082 | 0.656 |
| Rpim | 0.035 | 0.373 |
| Number of reflections | 208518 | 9027 |
| <I/σ(I)> | 13.9 | 1.9 |
| Completeness [%] | 98.1 | |
| Redundancy | 5 | |
| CC(1/2) | 0.998 | 0.686 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH 8.5, 0.1 M MgCl2 or Mg(CH3CO2)2, and 12-15% w/v PEG 20K or 12% w/v PEG 8K |
