7AAR

sugar/H+ symporter STP10 in inward open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	83.917, 99.005, 184.515
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.856 - 2.640
R-factor	0.2481
Rwork	0.247
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h7d
Data reduction software	DIALS (1.12)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	92.270	92.270	2.710
High resolution limit [Å]	2.640	11.810	2.640
Rmerge	0.123	0.053	1.297
Rmeas	0.134	0.062	1.405
Rpim	0.052	0.030	0.535
Total number of observations	152793
Number of reflections	23016	289	1729
<I/σ(I)>	6.6
Completeness [%]	99.9	92.6	100
Redundancy	6.6	5.3	6.8
CC(1/2)	0.987	0.894	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	292.15	0.3M NaCl, 0.1M MgCl2, 0.1M Bicine, 36-43% PEG400