7AAN

Crystal structure of the F-BAR domain of PSTIPIP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	105
Detector technology	PIXEL
Collection date	2015-12-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99987
Spacegroup name	P 21 21 21
Unit cell lengths	48.334, 71.867, 204.589
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.290 - 2.140
R-factor	0.2105
Rwork	0.209
R-free	0.24050
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7aal
RMSD bond length	0.001
RMSD bond angle	0.293
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.295	2.332
High resolution limit [Å]	2.140	2.140
Rmerge	0.391	3.109
Rmeas	0.401	3.198
Rpim		0.736
Number of reflections	18546	927
<I/σ(I)>	7.5	1.4
Completeness [%]	46.1	10.4
Redundancy	19.1	17.7
CC(1/2)	0.996	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM Bis-Tris-propane (pH=6.0), 17% (w/v) PEG 3350, 150 mM sodium citrate