7AAL

Crystal structure of the F-BAR domain of PSTIPIP1, G258A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	105
Detector technology	PIXEL
Collection date	2015-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	48.191, 73.023, 205.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.620 - 1.970
R-factor	0.1879
Rwork	0.186
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wpe
RMSD bond length	0.001
RMSD bond angle	0.368
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.620	2.172
High resolution limit [Å]	1.970	1.974
Rmerge	0.130	1.477
Rmeas	0.130
Rpim	0.039
Number of reflections	30894	1545
<I/σ(I)>	13.1	1.6
Completeness [%]	59.4	12.1
Redundancy	12.3	9.9
CC(1/2)	0.998	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100 mM Bis-Tris-propane (pH 6.5), 15% (w/v) PEG 3350, 0.25 M sodium citrate