7A9R
Racemic compound of RNA duplexes.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.283 |
| Spacegroup name | P 1 |
| Unit cell lengths | 21.095, 25.955, 38.514 |
| Unit cell angles | 104.60, 96.80, 91.77 |
Refinement procedure
| Resolution | 36.700 - 2.455 |
| Rwork | 0.178 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zq9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.900 | 2.600 |
| High resolution limit [Å] | 2.455 | 2.455 |
| Rmeas | 0.171 | 0.497 |
| Number of reflections | 4734 | 591 |
| <I/σ(I)> | 4.09 | |
| Completeness [%] | 82.2 | 61.4 |
| Redundancy | 1.87 | |
| CC(1/2) | 0.978 | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M zinc acetate, 0.1 M cacodylate buffer, 18% w/v polyehtylene glycol 8000. |
