7A8M

rsGreen0.7-K206A-N205G in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	140.869, 140.869, 73.224
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.665 - 1.600
R-factor	0.2041
Rwork	0.202
R-free	0.24460
RMSD bond length	0.006
RMSD bond angle	0.856
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.870	46.870	1.630
High resolution limit [Å]	1.600	8.760	1.600
Rmerge	0.044	0.028	0.684
Rmeas	0.046	0.030	0.721
Rpim	0.014	0.010	0.225
Total number of observations	367014	2401	18615
Number of reflections	36684	251	1820
<I/σ(I)>	26.5	71.4	3.5
Completeness [%]	100.0	99	100
Redundancy	10	9.6	10.2
CC(1/2)	1.000	0.998	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MES pH 6.0 20 % PEG 2000 mme 10 mM BaCl2