7A8I
rsGreen0.7-K206A-H148S partially in the green-off state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.287, 97.877, 98.344 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.938 - 2.000 |
| R-factor | 0.1675 |
| Rwork | 0.164 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.921 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.940 | 48.940 | 2.050 |
| High resolution limit [Å] | 2.000 | 9.170 | 2.000 |
| Rmerge | 0.079 | 0.039 | 0.723 |
| Rmeas | 0.083 | 0.041 | 0.751 |
| Rpim | 0.023 | 0.012 | 0.204 |
| Total number of observations | 816370 | 7920 | 60535 |
| Number of reflections | 63498 | 758 | 4430 |
| <I/σ(I)> | 18.1 | 40.9 | 3.6 |
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 12.9 | 10.4 | 13.7 |
| CC(1/2) | 0.999 | 0.999 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Bis-tris pH 6.5 20 % PEG 5000 mme |
