7A8I

rsGreen0.7-K206A-H148S partially in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	96.287, 97.877, 98.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.938 - 2.000
R-factor	0.1675
Rwork	0.164
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.921
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.940	48.940	2.050
High resolution limit [Å]	2.000	9.170	2.000
Rmerge	0.079	0.039	0.723
Rmeas	0.083	0.041	0.751
Rpim	0.023	0.012	0.204
Total number of observations	816370	7920	60535
Number of reflections	63498	758	4430
<I/σ(I)>	18.1	40.9	3.6
Completeness [%]	100.0	99.5	100
Redundancy	12.9	10.4	13.7
CC(1/2)	0.999	0.999	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Bis-tris pH 6.5 20 % PEG 5000 mme