7A8F

rsGreen0.7-K206A-F165L in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	H 3 2
Unit cell lengths	141.740, 141.740, 73.043
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.989 - 2.270
R-factor	0.1996
Rwork	0.196
R-free	0.25710
RMSD bond length	0.005
RMSD bond angle	0.780
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.990	46.990	2.340
High resolution limit [Å]	2.270	9.080	2.270
Rmerge	0.064	0.033	0.605
Rmeas	0.066	0.034	0.620
Rpim	0.015	0.008	0.133
Total number of observations	268648
Number of reflections	13100	228	1187
<I/σ(I)>	29
Completeness [%]	99.9	99.1	99.7
Redundancy	20.5	17.4	21.7
CC(1/2)	1.000	1.000	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM HEPES pH 7.5 25 % PEG 4000