7A89
rsGreen0.7-K206A-F145Q in the green-on state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-28 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | H 3 2 |
Unit cell lengths | 135.412, 135.412, 144.541 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.180 - 2.500 |
R-factor | 0.1809 |
Rwork | 0.178 |
R-free | 0.23430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.909 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX (1.11) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.180 | 48.180 | 2.600 |
High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
Rmerge | 0.061 | 0.031 | 0.706 |
Rmeas | 0.063 | 0.032 | 0.725 |
Rpim | 0.014 | 0.008 | 0.163 |
Total number of observations | 355351 | 7259 | 39370 |
Number of reflections | 17818 | 416 | 1989 |
<I/σ(I)> | 31.2 | 81.7 | 5.1 |
Completeness [%] | 100.0 | 99.4 | 99.9 |
Redundancy | 19.9 | 17.4 | 19.8 |
CC(1/2) | 0.999 | 0.999 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22 % PEG 4000 140 mM Mg(NO3)2 |