7A81

rsGreen0.7-K206A-F145A partially in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	140.895, 140.895, 72.652
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.721 - 1.700
R-factor	0.2012
Rwork	0.199
R-free	0.24820
RMSD bond length	0.007
RMSD bond angle	0.841
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.720	46.720	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.051	0.043	0.663
Rmeas	0.054	0.045	0.698
Rpim	0.017	0.015	0.217
Total number of observations	287315	2025	15892
Number of reflections	29944	231	1570
<I/σ(I)>	25	62.7	4
Completeness [%]	98.7	99.1	97.7
Redundancy	9.6	8.8	10.1
CC(1/2)	1.000	0.999	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000 4% 2,2,2-trifluoroethanol