7A7Y

rsGreen0.7-F145Q in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	47.197, 51.268, 48.583
Unit cell angles	90.00, 101.08, 90.00

Refinement procedure

Resolution	46.317 - 0.970
R-factor	0.1261
Rwork	0.125
R-free	0.14170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xow
Data reduction software	XDS
Data scaling software	Aimless (0.5.23)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.320	46.320	0.990
High resolution limit [Å]	0.970	5.310	0.970
Rmerge	0.064	0.060	0.457
Rmeas	0.076	0.071	0.594
Rpim	0.041	0.038	0.373
Total number of observations	405332	3000	8645
Number of reflections	129948	863	4118
<I/σ(I)>	10.9	22.3	1.8
Completeness [%]	96.8	99.7	62.3
Redundancy	3.1	3.5	2.1
CC(1/2)	0.995	0.985	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	200 mM KNO3 20 % PEG 3350