7A7V

rsGreen1-K206A partially in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 43 21 2
Unit cell lengths	73.550, 73.550, 114.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.343 - 2.000
R-factor	0.1611
Rwork	0.159
R-free	0.19360
RMSD bond length	0.007
RMSD bond angle	0.876
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.343	47.340	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.053	0.026	0.616
Rmeas	0.054	0.027	0.628
Rpim	0.011	0.006	0.120
Number of reflections	21948	314	1585
<I/σ(I)>	38.5
Completeness [%]	100.0	99.6	100
Redundancy	25.8	19.6	27.2
CC(1/2)	1.000	1.000	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Bis-tris pH 5.5 200 mM (NH4)2SO4 16 % PEG 8000