7A7U

rsGreen1 in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 43 21 2
Unit cell lengths	73.243, 73.243, 114.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.204 - 2.150
R-factor	0.1616
Rwork	0.160
R-free	0.20120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xow
RMSD bond length	0.007
RMSD bond angle	0.925
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.204	47.200	2.220
High resolution limit [Å]	2.150	8.860	2.150
Rmerge	0.059	0.031	0.581
Rmeas	0.060	0.031	0.593
Rpim	0.012	0.007	0.115
Number of reflections	17685	319	1499
<I/σ(I)>	36.6
Completeness [%]	100.0	99.4	99.9
Redundancy	25.1	18.4	26.4
CC(1/2)	1.000	1.000	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.0 M (NH4)2SO4 200 mM NaCl 100 mM Na-cacodylate pH 6.5