7A7N

rsGreen0.7b in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	31.866, 61.568, 105.117
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.974 - 1.200
R-factor	0.1392
Rwork	0.138
R-free	0.17040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.016
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.974	39.970	1.220
High resolution limit [Å]	1.200	6.570	1.200
Rmerge	0.046	0.025	0.422
Rmeas	0.054	0.029	0.492
Rpim	0.028	0.015	0.249
Number of reflections	63701	467	2967
<I/σ(I)>	14.5
Completeness [%]	97.4	97.8	92.7
Redundancy	3.6	3.4	3.6
CC(1/2)	0.999	0.999	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MMT pH 5.0 25 % PEG 1500