7A7M

rsGreen0.7b in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.8856
Spacegroup name	P 21 21 21
Unit cell lengths	32.016, 61.191, 105.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.990 - 1.600
R-factor	0.1648
Rwork	0.163
R-free	0.19770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xow
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.990	39.990	1.630
High resolution limit [Å]	1.600	8.760	1.600
Rmerge	0.052	0.034	0.589
Rmeas	0.059	0.041	0.675
Rpim	0.028	0.021	0.323
Total number of observations	119169	712	5597
Number of reflections	27495	203	1321
<I/σ(I)>	15.4	29.7	2.3
Completeness [%]	97.7	94.6	99
Redundancy	4.3	3.5	4.2
CC(1/2)	0.998	0.995	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	150 mM KBr 30 % PEG 2000 mme